Cytology Room(room 305)

The cytology chamber mainly consists of two enzyme markers and flow cytometry. The enzyme marker can detect the absorbance (Abs), fluorescence intensity (FL), and chemiluminescence (Lum) of horticultural plants. Flow cytometry can quantitatively determine many important parameters such as cell DNA content, cell volume, protein content, enzyme activity, cell membrane receptors, and surface antigens.

Full-Wavelength Multifunctional Enzyme Labeling Instrument

Basic information:

Model： Infinite M200PRO

Manufacturer： TECAN (Switzerland) Company

Device Configuration:

1. 
   

   Ultraviolet visible wavelength range: 200-1000 nm

   
   

   Fluorescence intensity：360—850nm，it has light absorption, fluorescence intensity, time-resolved fluorescence analysis, fluorescence polarization, FRET and BRET technology, function of chemiluminescence, and supports whole wavelength scanning

   
   

   Three separate detectors： function of fluorescence readings/chemiluminescence/light absorption are four gratings.

   
   

   Temperature control: 4-50 ℃; It has a shocking temperature equalizing incubation function

   
   

   With double syringes： FLASH chemiluminescence detection can be performed

   
   

   ATP concentration detection: glow and flash

   
   

   Read the plate type: 6-1536 board

Test Project：

1. 
   

   The DNA/RNA Quantitative Testing

   
   

   Research on Ion Channels

   
   

   Research on Ion Current

   
   

   Calcium Ion Testing

   
   

   Cell Vitality Testing

   
   

   The Analysis of Reporter Genes and Gene Expression

   
   

   Enzymology

   
   

   Research of Molecular Interactions and Molecular Association

   
   

   Quantitative Detection of Protein.

   
   

   TR-FRET/HTRF Application

   
   

   Cell Toxicological Research

   
   

   ELISA

   
   

   Immunological Analysis

   
   

   Fluorescence and Chemiluminescence Application, etc.

   
   

Multi-Marking Microporous Plate detection system

Basic information:

Model： I3X

Manufacturer： Molecular Devices (Austria) Company

Device configuration:

1．Detection functions: optical absorption, fluorescence, chemiluminescence, time-resolved fluorescence, alpha screen detection.

2．Four grating technology

3．Light: High-power xenon flash + high-energy LED lights

4．Detector: PMT (double PMT detection technology)

Test Project：

1. 
   

   The DNA/RNA/Protein Quantitative and Purity Testing

   
   

   AlphaScreen Test

   
   

   The Analysis of Reporter Genes and Gene Expression

   
   

   TR-FRET/HTRF Application

   
   

   ELISAs/Enzymology Dynamic Detection

   
   

   Immunological Analysis

   
   

   Equipped with a Syringe for Fast Dynamic Detection, etc.

BD Accuri Flow Cytometry

Basic Information:

Model：BDAccuriC6 Plus

Manufacturer：American BD( Singapore) Company

Device Configuration:

1. 
   

   Detection function: fluorescence detection

   
   

   Single detection of cellular capacity: up to 1 million cells can be processed

   
   

   Fluorescence detection system: fixed light path, even if the transport does not need to adjust the light path

   
   

   Detector: 488 blue light solid-state laser and 640 red light solid-state laser. PMT as a fluorescence detector. The 488 laser excites three fluorescence channels, and the 640 laser excites a fluorescence channel

Test project：

1. 
   

   Plant Cell Cycle Analysis

   
   

   Plant Cell Ploidy Analysis

   
   

   Study on the C Value of Plant Cells

   
   

   Fluorescent Protein Determination

   
   

   Anti-Stress Study